Yes, it's from "The Social Network". It's a scene where Mark Z. is explaining to Eduardo how important it is that the servers stay up all the time.
Of course it was, as far as I know, ficitonalized in the first place, although it rings true (in context) to some extent. What I wonder is, how much is that true now? That is, how much downtime would FB have to experience for enough users to start leaving, to the point that it might prompt a serious exodus.